THE VASOPRESSIN RECEPTOR OF THE BLOOD-BRAIN-BARRIER IN THE RAT HIPPOCAMPUS IS LINKED TO CALCIUM SIGNALING

被引：20

作者：

HESS, J ^{[1
]}

JENSEN, CV ^{[1
]}

DIEMER, NH ^{[1
]}

机构：

[1] UNIV COPENHAGEN,INST NEUROPATHOL,PHARMABIOTEC,DK-1168 COPENHAGEN,DENMARK

来源：

NEUROSCIENCE LETTERS | 1991年 / 132卷 / 01期

关键词：

VASOPRESSIN RECEPTOR; V1-SUBTYPE; BLOOD BRAIN BARRIER; CEREBRAL ENDOTHELIUM; HIPPOCAMPUS; FURA-2;

D O I：

10.1016/0304-3940(91)90419-T

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The signal transduction system of the vasopressin receptor in cerebral microvessels is not known but appears not to be adenylate cyclase/cyclic AMP. We determined the effect of arginine vasopressin (AVP) on the intracellular free calcium concentration [Ca2+]i in endothelial cells of isolated hippocampal microvessels of rats, using the fura-2 fluorescence technique. AVP administration caused a rapid and transient rise of cytosolic free calcium which was absent after extracellular calcium was removed, and could be blocked with the vasopressin V1 receptor antagonist, d(CH2)5Tyr-(Me)AVP. The vasopressin V2 receptor agonist, 1-deamino-8,D-AVP, on the contrary, failed to affect the intracellular free calcium level, and was unable to inhibit the AVP-induced rise of [Ca2+]i in the preparation. Our results, therefore, demonstrate the presence of a calcium-signalling, i.e. V1 vasopressin receptor at the blood-brain barrier in the hippocampus of the rat.

引用

页码：8 / 10

页数：3

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