EXPRESSION OF INDUCIBLE NITRIC-OXIDE SYNTHASE BY NEURONS FOLLOWING EXPOSURE TO ENDOTOXIN AND CYTOKINE

被引：146

作者：

MINCGOLOMB, D ^{[1
]}

TSARFATY, I ^{[1
]}

SCHWARTZ, JP ^{[1
]}

机构：

[1] NCI,ABL,BETHESDA,MD 20892

来源：

BRITISH JOURNAL OF PHARMACOLOGY | 1994年 / 112卷 / 03期

关键词：

CEREBELLAR GRANULE CELLS; MACROPHAGE; CEREBELLUM; INTERFERON-GAMMA; LIPOPOLYSACCHARIDE;

D O I：

10.1111/j.1476-5381.1994.tb13136.x

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

In the CNS, nitric oxide (NO) has been implicated as both a mediator of neurotoxicity and a neuromodulator. The inducible NO synthase (iNOS), thought to mediate toxic effects of NO, has been attributed to glial cells in the CNS. We now report that cerebellar granule cell neurones can be stimulated by lipopolysaccharide and interferon-gamma to express iNOS in vitro, as demonstrated by reverse transcription-polymerase chain reaction and fluorescent in situ hybridisation. The expression of both constitutive NO synthase (cNOS) and iNOS by neurones suggests that NO has diverse functions in the brain, and supports the possibility that iNOS plays a role in neuronal damage and inflammation following activation of brain microglia and production of cytokines.

引用

页码：720 / 722

页数：3

共 10 条

[1] NITRIC-OXIDE MEDIATES GLUTAMATE NEUROTOXICITY IN PRIMARY CORTICAL CULTURES [J].