A SIMPLIFIED METHOD FOR CRYOPRESERVATION OF HEMATOPOIETIC STEM-CELLS WITH -80-DEGREES-C MECHANICAL FREEZER WITH DIMETHYL-SULFOXIDE AS THE SOLE CRYOPROTECTANT

被引：26

作者：

GALMES, A ^{[1
]}

BESALDUCH, J ^{[1
]}

BARGAY, J ^{[1
]}

MATAMOROS, N ^{[1
]}

MOREY, M ^{[1
]}

NOVO, A ^{[1
]}

SAMPOL, A ^{[1
]}

机构：

[1] HOSP SON DURETA,SERV HEMATOL,E-07014 PALMA DE MALLORCA,SPAIN

来源：

LEUKEMIA & LYMPHOMA | 1995年 / 17卷 / 1-2期

关键词：

CRYOPRESERVATION; CRYOPROTECTIVE AGENTS; HEMATOPOIETIC STEM CELLS; BONE MARROW TRANSPLANTATION;

D O I：

10.3109/10428199509051720

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A simplified method for cryopreservation was developed with 10% dimethylsulfoxide (DMSO) as the sole cryoprotectant without rate-controlled freezing. This method produced high recovery rate for mononucleated cells (87%) and elevated trypan blue viability (90%), Autologous peripheral blood stem cells (PBSCs) and bone marrow cells with plasma and 10% DMSO were frozen and stored in a -80 degrees C mechanical freezer. Eleven patients with solid and hematological malignancies were transplanted with autologous bone marrow or PBSCs. The median number of infused mononuclear cells (MNC) and CD34+ cells were 3.63 x 10(8)/Kg and 4.80 x 10(6)/Kg, respectively. The median number of infused post-thawing CFU-GM was 20 x 10(4)/Kg. All patients showed a rapid and sustained engraftment. The mean times to reach a neutrophil count of 0.5 x 10(9)/L and a platelet count of 50 x 10(9)/L were 11 and 13 days, respectively. All patients are alive and 10 in unmaintained complete remission for 3-9 months after transplantation. These results show the efficacy of this simplified cryopreservation technique that will be useful for institutions without rate-controlled freezing facilities.

引用

页码：181 / 184

页数：4

共 11 条

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