CONTINUOUS DOSIMETRY OF THE BIOLOGICALLY HARMFUL UV-RADIATION IN ANTARCTICA WITH THE BIOFILM TECHNIQUE

被引:45
作者
QUINTERN, LE
PUSKEPPELEIT, M
RAINER, P
WEBER, S
ELNAGGAR, S
ESCHWEILER, U
HORNECK, G
机构
[1] GERMAN WORKING GRP POLAR MED, D-27574 BREMERHAVEN, GERMANY
[2] UNIV INNSBRUCK, INST METEOROL & GEOPHYS, A-6020 INNSBRUCK, AUSTRIA
[3] UNIV MAINZ, INST PHYS ATMOSPHERE, D-55128 MAINZ, GERMANY
[4] ALFRED WEGENER INST POLAR & MARINE RES, D-27570 BREMERHAVEN, GERMANY
[5] GERMAN AEROSP RES ESTAB DLR, INST AEROSP MED, D-51147 COLOGNE, GERMANY
关键词
BIOLOGICAL DOSIMETRY; BACILLUS-SUBTILIS SPORES; SOLAR UV RADIATION; OZONE HOLE; BIOFILM; BIOLOGICALLY EFFECTIVE DOSE; SOLAR RADIATION;
D O I
10.1016/1011-1344(93)06954-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
For the first time, a continuous biological dosimetry experiment for cytotoxic solar UV-radiation has been performed in Antarctica. The biologically harmful UV-radiation on the ground was measured at the German Antarctic Georg von Neumayer Station (70-degrees-37' S, 80-degrees-22' W) from December 1990 to March 1992 using the biofilm technique. The UV-sensitive targets were dried spores of Bacillus subtilis which were immobilized on the film surface. The UV-induced inhibition of biological activity, determined photometrically from the protein synthesized after incubation and staining, was taken as a measure for the absorbed UV-dose. Films were exposed in horizontal position for time intervals ranging from 4 days during summer up to 51 and 41 days before and after the polar night respectively. The use of different cut-off filters allowed the calculation of the biologically effective UVA, UVB and the complete UV-radiation (UVA+B). The data were compared with the global radiation and the ozone column thickness indicating an increase of biologically harmful UVB radiation during austral spring at reduced ozone concentrations yielding a radiation amplification factor (RAF) of 1.4, whereas for the total UV(A+B) range the RAF amounted to 0.3.
引用
收藏
页码:59 / 66
页数:8
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