MOUSE EMBRYONIC STEM-CELLS EXHIBIT HIGH-LEVELS OF EXTRACHROMOSOMAL HOMOLOGOUS RECOMBINATION IN A CHLORAMPHENICOL ACETYLTRANSFERASE ASSAY SYSTEM

被引：11

作者：

JASIN, M ^{[1
]}

LIANG, F ^{[1
]}

机构：

[1] CORNELL UNIV, GRAD SCH MED SCI, NEW YORK, NY 10021 USA

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 25期

关键词：

D O I：

10.1093/nar/19.25.7171

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mouse embryonic stem (ES) cells were compared to COS1 and CV1 cells for their ability to perform extrachromosomal homologous recombination. RSVCAT plasmid substrates consisting of overlapping chloramphenicol acetyltransferase (CAT) gene fragments were transiently transfected into cells and extracts were assayed for CAT activity. Approximately 10% activity, relative to transfection with a complete CAT gene, was recovered for the recombination substrates in each of the cell lines tested. ES cells, therefore, as other cell lines, are capable of high levels of extrachromosomal recombination.

引用

页码：7171 / 7175

页数：5

共 37 条

[1] RECOMBINATION OF HOMOLOGOUS DNA FRAGMENTS TRANSFECTED INTO MAMMALIAN-CELLS OCCURS PREDOMINANTLY BY TERMINAL PAIRING [J].