GROWING TIPS OF TYPE-I COLLAGEN FIBRILS FORMED INVITRO ARE NEAR-PARABOLOIDAL IN SHAPE, IMPLYING A RECIPROCAL RELATIONSHIP BETWEEN ACCRETION AND DIAMETER

被引:83
作者
HOLMES, DF
CHAPMAN, JA
PROCKOP, DJ
KADLER, KE
机构
[1] UNIV MANCHESTER,SCH MED,DEPT BIOCHEM,OXFORD RD,MANCHESTER M13 9PT,LANCS,ENGLAND
[2] THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,JEFFERSON INST MOLEC MED,DEPT BIOCHEM & MOLEC BIOL,PHILADELPHIA,PA 19107
[3] UNIV MANCHESTER,SCH MED,DEPT MOLEC BIOL & MED BIOPHYS,MANCHESTER M13 9PT,LANCS,ENGLAND
基金
英国惠康基金;
关键词
D O I
10.1073/pnas.89.20.9855
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Collagen fibrils generated in vitro at 37-degrees-C by enzymic removal of C-terminal propeptides from type I pC-collagen (an intermediate in the normal processing of type I procollagen to collagen containing the C-terminal propeptides but not the N-terminal propeptides) display shape polarity, with one tip fine tapered and the other coarse tapered. Mass measurements by scanning transmission electron microscopy show that the mass per unit length along both kinds of tip increases roughly linearly over distances of almost-equal-to 100 D periods from the fibril end [D (axial periodicity) = 67 nm]. The fine tips of fibrils of widely differing lengths exhibit near-identical mass distributions, the mass in all cases increasing at the rate of almost-equal-to 17 molecules per D period, irrespective of fibril length. Coarse tips display less regular behavior. These results show that (i) the shape of a fine tip is not conical but resembles more closely a paraboloid of revolution, and (ii) for this shape to be maintained throughout growth, accretion (rate of mass uptake per unit area) cannot everywhere be the same on the surface of the tip but must decrease as the diameter increases. To a first approximation, accretion is-proportional-to (diameter)-1.
引用
收藏
页码:9855 / 9859
页数:5
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