FERTILIZATION ANTIGEN (FA-1) COMPLETELY BLOCKS HUMAN SPERM BINDING TO HUMAN ZONA-PELLUCIDA - FA-1 ANTIGEN MAY BE A SPERM RECEPTOR FOR ZONA-PELLUCIDA IN HUMANS

被引：28

作者：

KADAM, AL

FATEH, M

NAZ, RK

机构：

[1] YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT OBSTET & GYNECOL,REPROD IMMUNOL & MOLEC BIOL LABS,BRONX,NY 10461

[2] NEW YORK FERTIL INST,NEW YORK,NY

来源：

JOURNAL OF REPRODUCTIVE IMMUNOLOGY | 1995年 / 29卷 / 01期

关键词：

SPERM ANTIGENS; ANTISPERM ANTIBODIES; ZONA PELLUCIDA; SPERM-ZONA INTERACTION; IMMUNOCONTRACEPTION;

D O I：

10.1016/0165-0378(95)00928-E

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The effects of purified human sperm fertilization antigen-1 (FA-1), affinity-purified monoclonal Fab' antibody to FA-1, and monoclonal Fab' antibody to phosphotyrosine residues on human sperm-zona interaction were investigated. The purified FA-1 antigen completely blocked sperm binding to zona pellucida (P < 0.0001). Also, the monoclonal Fab' antibodies to FA-1 antigen and phosphotyrosine residues significantly (P < 0.05) reduced sperm-zona pellucida interaction. Effects were observed when FA-1 antigen was preincubated with zonae pellucidae and the antibodies were preincubated with sperm before insemination and not vice versa. These results suggest that the tyrosine phosphorylation especially of FA-1 antigen has an important role in zona pellucida receptor recognition and binding. These findings also suggest that FA-1 antigen may be the sperm receptor involved in zona pellucida binding in humans.

引用

页码：19 / 30

页数：12

共 26 条

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