IMPROVED PROCEDURE FOR DIFFERENTIAL DISPLAY OF TRANSCRIPTS FROM COTTON TISSUES

被引：14

作者：

SONG, P ^{[1
]}

YAMAMOTO, E ^{[1
]}

ALLEN, RD ^{[1
]}

机构：

[1] TEXAS TECH UNIV,DEPT BIOL SCI,PLANT MOLEC BIOL LAB,LUBBOCK,TX 79401

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1995年 / 13卷 / 02期

关键词：

MESSENGER-RNA DIFFERENTIAL DISPLAY; POLYMERASE CHAIN REACTION; GOSSYPIUM HIRSUTUM; COTTON FIBER;

D O I：

10.1007/BF02668789

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved procedure for the differential display of mRNA from cotton tissues (Gossypium hirsutum L.) was developed. Total RNA was purified from immature cotton fibers and stripped ovules (fibers removed) and treated with DNase I. First strand cDNA was synthesized using anchored oligo-dT primers and reverse transcriptase. Use of alpha-[P-33]-dATP in pCR amplification of cDNAs produced the same band pattern as alpha-[S-35]-dATP but increased the sensitivity of differential display and resolution of differentially displayed bands. Reduction of dNTP concentrations also improved the resolution of differentially displayed bands. This procedure has been successfully used to isolate cDNA probes for genes expressed during cotton fiber development.

引用

页码：174 / 181

页数：8

共 10 条

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