CLONING AND EXPRESSION ANALYSIS OF A HUMAN CDNA HOMOLOGOUS TO XENOPUS TFIIIA

被引：22

作者：

DREW, PD

NAGLE, JW

CANNING, RD

OZATO, K

BIDDISON, WE

BECKER, KG

机构：

[1] NINCDS,NEUROIMMUNOL BRANCH,MOLEC IMMUNOL SECT,BETHESDA,MD 20892

[2] NINCDS,NEUROGENET SECT,BETHESDA,MD 20892

[3] NICHHD,MOLEC GROWTH REGULAT LAB,BETHESDA,MD 20892

来源：

GENE | 1995年 / 159卷 / 02期

关键词：

BASAL TRANSCRIPTION FACTOR; DNA-BINDING PROTEIN; RNA-BINDING PROTEIN; EVOLUTION;

D O I：

10.1016/0378-1119(95)00145-V

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We report here the nucleotide sequence of a clone, C2H2-34.10, isolated from a human brain cDNA library using degenerate oligodeoxyribonucleotide hybridization. C2H2-34.10 has extensive homology to the Xenopus laevis 5S DNA/RNA-binding protein, TFIIIA. The deduced amino acid (aa) sequence of the human clone gives a protein of 363 aa with identity to TFIIIA from both X. laevis (57%) and Rana pipiens (59%), This human clone contains nine C2H2-type zinc fingers like frog TFIIIA, Northern blot analysis indicates that the C2H2-34.10 RNA is expressed in human ovary, as well as human neuronal cell lines.

引用

页码：215 / 218

页数：4

共 28 条

[1] RNA PROTEIN INTERACTIONS OF STORED 5S-RNA WITH TFIIIA AND RIBOSOMAL PROTEIN-L5 DURING XENOPUS-OOGENESIS [J].