HEAT-SHOCK PROTEINS EXPRESSED ON THE SURFACE OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE I-INFECTED CELL-LINES INDUCE AUTOANTIBODIES IN RABBITS

被引：38

作者：

CHOUCHANE, L ^{[1
]}

BOWERS, FS ^{[1
]}

SAWASDIKOSOL, S ^{[1
]}

SIMPSON, RM ^{[1
]}

KINDT, TJ ^{[1
]}

机构：

[1] NIAID,IMMUNOGENET LAB,ROCKVILLE,MD 20852

来源：

JOURNAL OF INFECTIOUS DISEASES | 1994年 / 169卷 / 02期

关键词：

D O I：

10.1093/infdis/169.2.253

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Eight human T cell leukemia virus type I (HTLV-I)-infected cell lines were derived in vitro from peripheral blood mononuclear cells of 8 rabbits. Each rabbit was then inoculated with its own HTLV-I-transformed cells, after which all but 1 rabbit had anti-heat-shock protein (hsp) antibodies in sera. Cell line RH/K34, which failed to raise a response to hsp70, caused lethal leukemia when >2 X 10(8) live cells were injected into unrelated outbred rabbits. Rabbits injected with cell-free virus isolated from RH/K34 cells produced anti-hsp70 antibodies and became infected but developed no fatal disease. ELISA inhibition and flow cytometry analyses indicated that hsp molecules are expressed on the surface of RH/K34 and RH/K30, a nonlethal HTLV-I cell line used for comparison; surface hsp expression does not occur normally. Two proteins of similar to 72 and 93 kDa were detected by Western blot in extracts of RH/K30 cells. Presence of anti-hsp70 antibodies correlated with resistance to lethal doses of live RH/K34 cells, suggesting that hsp immunity may influence the outcome of RH/K34 pathogenicity.

引用

页码：253 / 259

页数：7

共 38 条

[1] HEAT-SHOCK AND THE HEAT-SHOCK PROTEINS [J].