LOCALIZATION OF METALLOTHIONEIN ISOFORM MESSENGER-RNAS IN RAT HEPATOMA (H4) CELLS

被引：17

作者：

MAHON, P

BEATTIE, J

GLOVER, LA

HESKETH, J

机构：

[1] ROWETT RES INST, DIV BIOCHEM SCI, BUCKSBURN AB2 9SB, ABERDEEN, SCOTLAND

[2] UNIV ABERDEEN, MARISCHAL COLL, DEPT MOLEC & CELL BIOL, ABERDEEN AB9 1AS, SCOTLAND

来源：

FEBS LETTERS | 1995年 / 373卷 / 01期

关键词：

CYTOSKELETON; POLYSOME; MESSENGER-RNA; METALLOTHIONEIN; METAL; COMPARTMENTATION;

D O I：

10.1016/0014-5793(95)01000-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The localisation of metallothionein isoform mRNAs in rat hepatoma (H4) cells was investigated using two approaches, namely Northern hybridisation of total RNA extracted from free, cytoskeletal-bound and membrane-bound polysomes isolated by a sequential detergent/salt extraction procedure and in situ hybridisation. The cytoskeletal-bound polysomes mere enriched in metallothionein-I (MT-I) and c-myc mRNAs but showed a significantly lon er enrichment in MT-II mRNA. These findings indicate that the MT-I mRNA is localised to the cytoskeleton during translation. In situ hybridisation using a biotin-labelled oligonucleotide probe revealed a predominantly perinuclear localisation for the MT-I mRNA.

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页码：76 / 80

页数：5

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