ALZHEIMER BRAIN PROTEINS INVESTIGATED BY 2-DIMENSIONAL GEL-ELECTROPHORESIS WITH IMMOBILIZED PH GRADIENTS IN THE FIRST-DIMENSION

被引：9

作者：

MATTILA, KM

FREY, H

机构：

[1] Department of Clinical Medicine, University of Tampere

来源：

ELECTROPHORESIS | 1994年 / 15卷 / 05期

关键词：

D O I：

10.1002/elps.1150150199

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two-dimensional (2-D) gel electrophoresis with immobilized pH gradient 4-8 in the first dimension was applied in the analysis of Alzheimer's disease brain proteins. The silver-stained 2-D maps of extracts from the frontal cerebral cortex were examined. About 800 and 550 protein spots could be observed on the electrophoretograms from the total and buffer-soluble fractions, respectively. In comparing the gels, four protein spots could be seen in all Alzheimer 2-D patterns which were either undetectable (one spot) or significantly weaker (two spots) or stronger (one spot) in density than those from the controls.

引用

页码：721 / 725

页数：5

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