IN-VITRO EVOLUTION OF NEW RIBOZYMES WITH POLYNUCLEOTIDE KINASE-ACTIVITY

被引：215

作者：

LORSCH, JR

SZOSTAK, JW

机构：

[1] HARVARD UNIV, DEPT BIOCHEM & MOLEC BIOL, CAMBRIDGE, MA 02138 USA

[2] HARVARD UNIV, SCH MED, DEPT GENET, BOSTON, MA 02115 USA

来源：

NATURE | 1994年 / 371卷 / 6492期

关键词：

D O I：

10.1038/371031a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have isolated a large number of polynucleotide kinase ribozymes from a pool of RNA molecules consisting of an ATP-binding domain flanked by regions of random sequence. Different classes of kinases catalyse the transfer of the gamma-thiophosphate of ATP-gamma S to the 5'-hydroxyl or to internal 2'-hydroxyls. An engineered version of one class is able to catalyse the transfer of thiophosphate from ATP-gamma S to the 5'-hydroxyl of an exogenous oligoribonucleotide substrate with multiple turnover, thus acting as a true enzyme.

引用

页码：31 / 36

页数：6

共 39 条

[1] [Anonymous], 1985, ENZYME STRUCTURE MEC
[2] HIV-1 REV REGULATION INVOLVES RECOGNITION OF NON-WATSON-CRICK BASE-PAIRS IN VIRAL-RNA
BARTEL, DP
ZAPP, ML
GREEN, MR
SZOSTAK, JW
[J]. CELL, 1991, 67 (03) : 529 - 536
[3] ISOLATION OF NEW RIBOZYMES FROM A LARGE POOL OF RANDOM SEQUENCES
BARTEL, DP
SZOSTAK, JW
[J]. SCIENCE, 1993, 261 (5127) : 1411 - 1418
[4] SPECIFIC INTERACTION BETWEEN THE SELF-SPLICING RNA OF TETRAHYMENA AND ITS GUANOSINE SUBSTRATE - IMPLICATIONS FOR BIOLOGICAL CATALYSIS BY RNA
BASS, BL
CECH, TR
[J]. NATURE, 1984, 308 (5962) : 820 - 826
[5] DIRECTED EVOLUTION OF AN RNA ENZYME
BEAUDRY, AA
JOYCE, GF
[J]. SCIENCE, 1992, 257 (5070) : 635 - 641
[6] SELECTION OF SINGLE-STRANDED-DNA MOLECULES THAT BIND AND INHIBIT HUMAN THROMBIN
BOCK, LC
GRIFFIN, LC
LATHAM, JA
VERMAAS, EH
TOOLE, JJ
[J]. NATURE, 1992, 355 (6360) : 564 - 566
[7] RELATIVE REACTIVITIES OF P-NITROPHENYL PHOSPHATE AND PHOSPHOROTHIOATE TOWARD ALKALINE PHOSPHATASE AND IN AQUEOUS HYDROLYSIS
BRESLOW, R
KATZ, I
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1968, 90 (26) : 7376 - &
[8] Cadwell R C, 1992, PCR Methods Appl, V2, P28, DOI 10.1101/gr.2.1.28
[9] NOVEL GUANOSINE REQUIREMENT FOR CATALYSIS BY THE HAIRPIN RIBOZYME
CHOWRIRA, BM
BERZALHERRANZ, A
BURKE, JM
[J]. NATURE, 1991, 354 (6351) : 320 - 322
[10] 3 SMALL RIBOOLIGONUCLEOTIDES WITH SPECIFIC ARGININE SITES
CONNELL, GJ
ILLANGESEKARE, M
YARUS, M
[J]. BIOCHEMISTRY, 1993, 32 (21) : 5497 - 5502

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