DISTRIBUTION OF E-CADHERIN AND EP-CAM IN THE HUMAN LUNG DURING DEVELOPMENT AND AFTER INJURY

被引：49

作者：

KASPER, M ^{[1
]}

BEHRENS, J ^{[1
]}

SCHUH, D ^{[1
]}

MULLER, M ^{[1
]}

机构：

[1] MAX DELBRUCK CTR MOLEC MED,D-13125 BERLIN,GERMANY

来源：

HISTOCHEMISTRY AND CELL BIOLOGY | 1995年 / 103卷 / 04期

关键词：

D O I：

10.1007/BF01457412

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Paraffin sections were obtained of human fetal, adult, and pathological lung (pulmonary fibrosis after radiotherapy or chemotherapy). The localization of epithelial adhesion molecules E-cadherin and Ep-CAM (former epithelial surface 40 kDa glycoprotein) was investigated by immunoperoxidase and/or immunofluorescence techniques with monoclonal antibodies. During development, the epithelia of the primary pulmonary primordium, the secondary bronchi and the adult bronchial epithelium retained immunoreactivity for E-cadherin and EP-CAM with lateral immunostaining of cell membranes. In normal adult lungs, Ep-CAM was detected in type I and II alveolar epithelial cells, whereas E-cadherin was confined to the basolateral domain of type II cells. In pulmonary fibrosis, Ep-CAM could be further detected on the cell surface of epithelial remnants. In contrast, E-cadherin expression was characterized by a change of the membrane localization to a spotty, cytoplasmic pattern in the alveolar epithelium, possibly indicating functional inactivation of the protein during fibrogenesis.

引用

页码：281 / 286

页数：6

共 35 条

[1] RELATIONSHIP OF MESENCHYMAL CHANGES TO ALVEOLAR EPITHELIAL-CELL DIFFERENTIATION IN FETAL-RAT LUNG [J].