METHYLATED BLOCKED 5' TERMINAL SEQUENCES OF SEA-URCHIN EMBRYO MESSENGER-RNA CLASSES CONTAINING AND LACKING POLY(A)

被引：39

作者：

SURREY, S ^{[1
]}

NEMER, M ^{[1
]}

机构：

[1] INST CANC RES, PHILADELPHIA, PA 19111 USA

来源：

CELL | 1976年 / 9卷 / 04期

关键词：

D O I：

10.1016/0092-8674(76)90041-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sea urchin embryo mRNA of 3 distinct classes, histone mRNA, nonhistone mRNA containing poly(A), "[+A] mRNA," and nonhistone mRNA lacking poly(A), ''''[-A] mRNA'''', all contain blocked 5'' terminal sequences in which 7-methylguanosine is linked 5''-5'' via a triphosphate bridge to a 2''-0-methylated nucleotide. Only 1 general type of 5'' terminal structure, 7mGpppXmpYp, is present. Additional 2''-0-methylation in the Y residue has not been found either in early or late stage embryos. A substantial proportion of the polyribosomal mRNA in all 3 classes contain this blocked structure. Whereas both classes of nonhistone mRNA have internal base methylations, histone mRNA lack such modifications.

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页码：589 / 595

页数：7

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