PROTOPLAST INDUCTION IN MICRASTERIAS AND COSMARIUM

被引：19

作者：

BERLINER, MD ^{[1
]}

WENC, KA ^{[1
]}

机构：

[1] SIMMONS COLL, DEPT BIOL, BOSTON, MA 02115 USA

来源：

PROTOPLASMA | 1976年 / 89卷 / 3-4期

关键词：

D O I：

10.1007/BF01275756

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Protoplasts of the desmids M. angulosa, M. denticulata, M. thomasiana and C. turpinii were obtained by incubating cells in Waris'' liquid medium + 0.3 M mannitol + 2% Cellulysin for 1-3 h. One osmotically fragile protoplast was formed at the isthmus from the joint contents of both semicells. The resultant protoplasts were bright green and remained so for more than 5 days in the osmotically protective medium. The protoplast yield was better than 80%. The empty cell walls were not digested by the Cellulysin or by autolytic enzymes.

引用

页码：389 / 393

页数：5

共 13 条

[1]

BERLINER M D, 1970, Science (Washington D C), V167, P1255, DOI 10.1126/science.167.3922.1255

[2] INDUCTION OF PROTOPLASTS OF SCHIZOSACCHAROMYCES-OCTOSPORUS BY MAGNESIUM SULFATE AND 2-DEOXY-D-GLUCOSE [J].