REGULATION OF VIOLAXANTHIN DE-EPOXIDASE ACTIVITY BY PH AND ASCORBATE CONCENTRATION

被引：148

作者：

BRATT, CE

ARVIDSSON, PO

CARLSSON, M

AKERLUND, HE

机构：

[1] Plant Biochemistry, Lund University, Lund, S-221 00

来源：

PHOTOSYNTHESIS RESEARCH | 1995年 / 45卷 / 02期

关键词：

VIOLAXANTHIN DE-EPOXIDASE; SPINACH; XANTHOPHYLL CYCLE; PH DEPENDENCE; ASCORBATE;

D O I：

10.1007/BF00032588

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The activity of violaxanthin de-epoxidase has been studied both in isolated thylakoids and after partial purification, as a function of pH and ascorbate concentration. We demonstrate that violaxanthin de-epoxidase has a K-m for ascorbate that is strongly dependent on pH, with values of 10, 2.5, 1.0 and 0.3 mM at pH 6.0, 5.5, 5.0 and 4.5, respectively. These values can be expressed as a single K-m +/- 0.1 +/- 0.02 mM for the acid form of ascorbate. Release of the protein from the thylakoids by sonication was also found to be strongly pH dependent with a cooperativity of 4 with respect to protons and with an inflexion point at pH 6.7. These results can explain some of the discrepancies reported in the literature and provide a more consistent view of zeaxanthin formation in vivo.

引用

页码：169 / 175

页数：7

共 24 条

[1]

Ada W.W., Demmig-Ada B., Verhoeven A.S., Barker D.H., Photoinhibition during winter stress: Involvement of sustained xanthophyll cycle-dependent energy dissipation, Aust J Plant Physiol, 22, pp. 261-276, (1994)

[2]

Bjorkman O., Demmig-Ada B., Regulation of photosynthetic light energy capture, conversion, and dissipation in leaves of higher plants, Ecophisiology of Photosynthesis, pp. 17-47, (1994)

[3]

Bolliger H.R., Konig A., Vitamins, including carotenoids, chlorophylls and biologically active quinones, Thin-layer Chromatography, pp. 259-311, (1969)

[4]

Briantais J-M, Vernotte C., Picaud M., Krause G.H., A quantitative study of the slow decline of chlorophyll a fluorescence in isolated chloroplasts, Biochim Biophys Acta, 548, pp. 128-138, (1979)

[5]

Demmig-Ada B., Ada W.W., Logan B.A., Verhoeven A.S., Xanthophyll cycle-dependent energy dissipation and flexible Photosystem II efficiency in plant acclimated to light stress, Aust J Plant Physiol, 22, pp. 249-260, (1995)

[6]

Demmig-Ada B., Winter K., Kruger A., Czygan F-C, Zeaxanthin and the induction and relaxation kinetics of the dissipation of excess excitation energy in leaves in 2% O<sub>2</sub>, 0% CO<sub>2</sub>, Plant Physiol, 90, pp. 887-893, (1989)

[7]

Demmig B., Winter K., Kruger A., Czygan F-C, Photoinhibition and zeaxanthin formation in intact leaves, PLANT PHYSIOLOGY, 84, pp. 218-224, (1987)

[8]

Foyer C.H., Ascorbic acid, Antioxidantsin Higher Plants, pp. 31-58, (1993)

[9]

Frank H.A., Cua A., Chynwat V., Young A., Gosztola D., Wasielewski, Photophysics of the carotenoids associated with the xanthophyll cycle in photosynthesis, Photosynth Res, 41, pp. 389-395, (1994)

[10]

Gillham D.J., Dodge A.D., Hydrogen-peroxide-scavenging systems within pea chloroplasts, Planta, 167, pp. 246-251, (1986)

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