PHYSICAL AND GENETIC-MAP OF THE NEISSERIA-GONORRHOEAE STRAIN MS11-N198 CHROMOSOME

被引：58

作者：

BIHLMAIER, A

ROMLING, U

MEYER, TF

TUMMLER, B

GIBBS, CP

机构：

[1] MAX PLANCK INST BIOL,INFEKT BIOL ABT,SPEMANNSTR 34,W-7400 TUBINGEN,GERMANY

[2] MED HSCH HANNOVER,ZENTRUM BIOCHEM,INST BIOPHYS CHEM,W-3000 HANNOVER 61,GERMANY

来源：

MOLECULAR MICROBIOLOGY | 1991年 / 5卷 / 10期

关键词：

D O I：

10.1111/j.1365-2958.1991.tb02099.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A macro-restriction map of the Neisseria gonorrhoeae chromosome was constructed using the enzymes NheI and SpeI. Combinations of one- and two-dimensional electrophoresis of completely or partially digested chromosomal DNA were performed to align the restriction fragments. The chromosome is circular, with an estimated size of 2.33 Mb +/- 35 kb. A genetic map was derived from the physical map; positions of over 60 defined loci were determined by Southern hybridization.

引用

页码：2529 / 2539

页数：11

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