STABILIZATION OF EGG PHOSPHATIDYLCHOLINE LIPOSOMES BY THE INSERTION OF SULFATIDE

被引：8

作者：

NODA, H ^{[1
]}

KURONO, M ^{[1
]}

OHISHI, N ^{[1
]}

YAGI, K ^{[1
]}

机构：

[1] INST APPL BIOCHEM, MITAKE, GIFU 50501, JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1153卷 / 01期

关键词：

LIPOSOME; PHOSPHATIDYLCHOLINE; SULFATIDE; MEMBRANE STABILITY; (EGG);

D O I：

10.1016/0005-2736(93)90284-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The characteristic effects of sulfatide and its derivatives on the stability of small unilamellar vesicles of egg phosphatidylcholine liposomes in phosphate-buffered saline were investigated by measuring the leakage of carboxyfluorescein that had been entrapped in these vesicles. We found that both the sulfate group and a long acyl chain, such as lignoceric acid, of the sulfatide are essential for the stabilization. The sulfatide derivatives that contain a somewhat shorter acyl chain such as stearic acid had no effect to suppress the leakage of carboxyfluorescein. The galactose residue of sulfatide is not essential to suppress the leakage. H-1-NMR study using a paramagnetic shift reagent demonstrated that the distribution of phosphatidylcholine in the vesicles containing sulfatide is homogeneous, which seems to contribute to the stability of the membrane.

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页码：127 / 131

页数：5

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