THE EFFECTS OF TEMPERATURE-ACCLIMATION ON THE PHOTOINHIBITORY RESPONSES OF ULVA-ROTUNDATA BLID

被引:10
作者
FRANKLIN, LA [1 ]
机构
[1] DUKE UNIV, MARINE LAB, BEAUFORT, NC 28516 USA
关键词
CHLOROPHYLL FLUORESCENCE; PHOTOINHIBITION; PHOTOPROTECTION; TEMPERATURE; ULVA; XANTHOPHYLL CYCLE;
D O I
暂无
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The effect of acclimation to 25, 18, or 10-degrees-C on the relationship between photoprotection and photodamage was tested in low-light-grown (80 mumol m-2.s-1) Ulva rotundata Blid. exposed to several higher irradiances at the acclimation temperature. Changes in chlorophyll fluorescence parameters (minimum fluorescence, F0, and the ratio of variable to maximum fluorescence, F(v)/F(m), measured after 5 min darkness) were monitored during 5 h transfers to 350, 850, and 1700 mumol. m-2 . S-1, and during recovery after 1- or 5-h treatments. At all temperatures, rate of onset and final extent of photoinhibition, measured by a decrease in F(v)/F(m), increased with increasing irradiance. At a given photoinhibitory irradiance, rate of onset was most rapid at 10-degrees-C, but the extent was temperature-independent. Recovery rates from mild light stress were similar at all temperatures, but recovery from the most extreme photoinhibitory treatment lagged 2 h at 10-degrees-C. De-epoxidation of xanthophyll-cycle components proceeded faster and to a lower epoxidation status at 25-degrees-C, but there was little difference in the pool size among the three growth conditions. Using chloramphenicol to inhibit chloroplast protein synthesis and dithiothreitol to inhibit violaxanthin de-epoxidation, it was shown that at the lowest light treatment given, the extent of photoinhibition could be attributed both to greater amounts of photodamage and to greater zeaxanthin-related photoprotection at 25 than at 10-degrees-C. While these two mechanisms for high-light-induced loss of photosynthetic efficiency were operating at 10-degrees-C, there was evidence for a relatively greater proportion of zeaxanthin-unrelated photoprotection at the low temperature. This photoprotective mechanism is related to a rapidly reversible increase in F0 and is insentivite to both chloramphenicol and dithiothreitol.
引用
收藏
页码:324 / 331
页数:8
相关论文
共 37 条
[1]  
[Anonymous], 1987, TOPICS PHOTOSYNTHESI
[2]   INVITRO STUDIES ON LIGHT-INDUCED INHIBITION OF PHOTOSYSTEM-II AND D1-PROTEIN DEGRADATION AT LOW-TEMPERATURES [J].
ARO, EM ;
HUNDAL, T ;
CARLBERG, I ;
ANDERSSON, B .
BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1019 (03) :269-275
[3]  
BILGER W, 1991, PLANTA, V184, P226, DOI [10.1007/BF01102422, 10.1007/BF00197951]
[4]  
CHOW WS, 1989, PHOTOSYNTH RES, V21, P17, DOI 10.1007/BF00047171
[5]   CAROTENOIDS AND PHOTOPROTECTION IN PLANTS - A ROLE FOR THE XANTHOPHYLL ZEAXANTHIN [J].
DEMMIGADAMS, B .
BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1020 (01) :1-24
[6]   PHOTOPROTECTION AND OTHER RESPONSES OF PLANTS TO HIGH LIGHT STRESS [J].
DEMMIGADAMS, B ;
ADAMS, WW .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1992, 43 :599-626
[7]  
FALK S, 1990, PHYSIOL PLANTARUM, V78, P173
[8]  
FRANKLIN LA, 1992, PLANTA, V186, P399, DOI 10.1007/BF00195321
[9]   RESOLUTION OF LUTEIN AND ZEAXANTHIN USING A NON-ENDCAPPED, LIGHTLY CARBON-LOADED C-18 HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC COLUMN [J].
GILMORE, AM ;
YAMAMOTO, HY .
JOURNAL OF CHROMATOGRAPHY, 1991, 543 (01) :137-145
[10]   EFFECT OF TEMPERATURE ON PHOTOINHIBITION OF PHOTOSYNTHESIS, RECOVERY, AND TURNOVER OF THE 32 KD CHLOROPLAST PROTEIN IN LEMNA-GIBBA [J].
GONG, H ;
NILSEN, S .
JOURNAL OF PLANT PHYSIOLOGY, 1989, 135 (01) :9-14