EXOGENOUS POLYAMINES IMPROVE ROOTING OF HAZEL MICROSHOOTS

被引:34
作者
REY, M [1 ]
DIAZSALA, C [1 ]
RODRIGUEZ, R [1 ]
机构
[1] UNIV OVIEDO,DEPT BIOL ORGANISMOS & SISTEMAS,FISIOL VEGETAL LAB,E-33005 OVIEDO,SPAIN
关键词
CORYLUS AVELLANA; MICROPROPAGATION; PUTRESCINE; SYNERGISTIC EFFECT; TISSUE CULTURE;
D O I
10.1007/BF00046087
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A strong positive effect of polyamines on rooting of microshoots of adult hazel (Corylus avellana L., cv. Gironell) is described. The effect of polyamines, both in the root induction solution and in the actual rooting medium, was assessed in order to study the effect on the successive rooting phases. Polyamines improved rooting of indole-3-butyric acid-treated microshoots in a synergistic fashion, perhaps by favouring a better induction of roots, with an acceleration of the response (only half the time required for rooting compared to the control). When applied without indole-3-butyric acid, polyamines had only a limited positive effect on rooting, although longer exposure times and/or higher concentrations could increase their effect. Possible rapid uptake and translocation of polyamines in the xylem in our system is discussed. The results offer a new approach to enhance rooting ability of species that are normally difficult to root.
引用
收藏
页码:303 / 308
页数:6
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