FURTHER EVIDENCE THAT MOST LUTEINIZING-HORMONE-RELEASING HORMONE NEURONS ARE NOT DIRECTLY ESTROGEN-RESPONSIVE - SIMULTANEOUS LOCALIZATION OF LUTEINIZING-HORMONE-RELEASING HORMONE AND ESTROGEN-RECEPTOR IMMUNOREACTIVITY IN THE GUINEA-PIG BRAIN

被引:73
作者
WATSON, RE
LANGUB, MC
LANDIS, JW
机构
[1] Department of Anatomy and Neurobiology, University of Kentucky Medical Center, Lexington, Kentucky
关键词
LUTEINIZING HORMONE-RELEASING HORMONE; PREOPTIC AREA; HYPOTHALAMUS; REPRODUCTION; STEROID;
D O I
10.1111/j.1365-2826.1992.tb00173.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Gonadotropin secretion from the pituitary is regulated in large part by steroid action on the brain. An important question concerns whether luteinizing hormone-releasing hormone (LHRH) neurons themselves transduce steroid signals, or whether, alternatively, steroid-sensitive interneuronal populations regulate their activity. A previous study in the rat employing steroid autoradiography combined with LHRH immunocytochemistry revealed that only an exceedingly small percentage of LHRH-immunoreactive (ir) neurons was estrogen concentrating. This study has examined the relationship of estrogen receptive and LHRH-ir cells in the male and female guinea-pig brain with double label immunocytochemistry. Since estrogen receptor-ir, as demonstrated with antibody H222, is known to be confined predominantly to the cell nucleus, whereas LHRH-ir is localized mainly in the cytoplasm, single cells can be double-labeled. Diaminobenzidine tetrahydrochloride was used for localization of LHRH-ir while nickel-enhanced diaminobenzidine tetrahydrochloride was used for localization of estrogen receptor-ir. The results revealed that there were many brain nuclei that contained both LHRH and estrogen receptor-positive cells, including the preventricular periventricular nucleus, the anterior-subcompact nucleus of the medial preoptic nucleus (MPNa), the remainder of the medial preoptic nucleus, the retrochiasmatic area, and the anterior, dorsomedial, ventrolateral and arcuate nuclei. However, of a total of 2,604 LHRH-ir cells that were examined, we observed only 5 double-labeled cells (<0.2%). The double-labeled cells were not restricted to a single nucleus; they were present in the MPNa, the retrochiasmatic area and the arcuate nucleus. Moreover, at the light microscopic level, LHRH cells quite frequently appeared to be apposed to estrogen receptor-positive cells (8.8% in the female), especially in the MPNa. These results lend further support to the notion that estrogen-mediated regulation of the LHRH system is achieved primarily through estrogen receptive interneurons. However, due to the existence of LHRH-LHRH synaptic interactions, the possibility also exists that a small population of estrogen-sensitive LHRH neurons could contribute to generalized activation of the LHRH system.
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页码:311 / 317
页数:7
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