AN AMINO-TERMINAL FRAGMENT OF SV40 T-ANTIGEN TRANSFORMS REF52 CELLS

被引：19

作者：

SOMPAYRAC, L

DANNA, KJ

机构：

[1] Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder

来源：

VIROLOGY | 1992年 / 191卷 / 01期

关键词：

D O I：

10.1016/0042-6822(92)90206-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An SV40 mutant, T147D, encodes only the amino-terminal 147 amino acids of large T antigen and does not make small t antigen. We show here that a retrovirus which expresses this mutant T antigen transforms rat REF52 cells as efficiently as a retrovirus that expresses both the wild-type large and small T antigens. This cell line had previously been refractory to transformation by mutants that make short, amino-terminal fragments of T antigen. © 1992.

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页码：439 / 442

页数：4

相关论文

共 22 条

[1] SEQUENCES FROM POLYOMAVIRUS AND SIMIAN VIRUS-40 LARGE T-GENES CAPABLE OF IMMORTALIZING PRIMARY RAT EMBRYO FIBROBLASTS [J].

ASSELIN, C ;

BASTIN, M .

JOURNAL OF VIROLOGY, 1985, 56 (03) :958-968

[2]

CHEN JD, 1992, ONCOGENE, V7, P1167

[3] A FRAGMENT OF THE SV40 LARGE T-ANTIGEN GENE TRANSFORMS [J].

CLAYTON, CE ;

MURPHY, D ;

LOVETT, M ;

RIGBY, PWJ .

NATURE, 1982, 299 (5878) :59-61

[4] MONOCLONAL-ANTIBODIES SPECIFIC FOR SIMIAN VIRUS-40 TUMOR-ANTIGENS [J].

HARLOW, E ;

CRAWFORD, LV ;

PIM, DC ;

WILLIAMSON, NM .

JOURNAL OF VIROLOGY, 1981, 39 (03) :861-869

[5] A NOVEL FORM OF TNF/CACHECTIN IS A CELL-SURFACE CYTO-TOXIC TRANSMEMBRANE PROTEIN - RAMIFICATIONS FOR THE COMPLEX PHYSIOLOGY OF TNF [J].

KRIEGLER, M ;

PEREZ, C ;

DEFAY, K ;

ALBERT, I ;

LU, SD .

CELL, 1988, 53 (01) :45-53

[6] THE P53 PROTEIN AND ITS INTERACTIONS WITH THE ONCOGENE PRODUCTS OF THE SMALL DNA TUMOR-VIRUSES [J].

LEVINE, AJ .

VIROLOGY, 1990, 177 (02) :419-426

[7] THE RETINOBLASTOMA SUSCEPTIBILITY GENE-PRODUCT UNDERGOES CELL CYCLE-DEPENDENT DEPHOSPHORYLATION AND BINDING TO AND RELEASE FROM SV40 LARGE-T [J].

LUDLOW, JW ;

SHON, J ;

PIPAS, JM ;

LIVINGSTON, DM ;

DECAPRIO, JA .

CELL, 1990, 60 (03) :387-396

[8] CONSTRUCTION OF A RETROVIRUS PACKAGING MUTANT AND ITS USE TO PRODUCE HELPER-FREE DEFECTIVE RETROVIRUS [J].

MANN, R ;

MULLIGAN, RC ;

BALTIMORE, D .

CELL, 1983, 33 (01) :153-159

[9] A FRAGMENT OF THE SIMIAN-VIRUS 40 EARLY GENOME CAN INDUCE TUMORS IN NUDE-MICE [J].

PAN, SH ;

SOMPAYRAC, LM ;

KNOWLES, BB ;

DANNA, KJ .

JOURNAL OF VIROLOGY, 1985, 53 (03) :988-989

[10] A DNA REPLICATION-POSITIVE MUTANT OF SIMIAN VIRUS-40 THAT IS DEFECTIVE FOR TRANSFORMATION AND THE PRODUCTION OF INFECTIOUS VIRIONS [J].

PEDEN, KWC ;

SPENCE, SL ;

TACK, LC ;

CARTWRIGHT, CA ;

SRINIVASAN, A ;

PIPAS, JM .

JOURNAL OF VIROLOGY, 1990, 64 (06) :2912-2921