EFFECTS OF CAMPTOTHECIN, AN INHIBITOR OF DNA TOPOISOMERASE-I ON RIBOSOMAL GENE STRUCTURE AND FUNCTION IN TG CELLS

被引：9

作者：

FARABEGOLI, F ^{[1
]}

GOVONI, M ^{[1
]}

NOVELLO, F ^{[1
]}

机构：

[1] UNIV BOLOGNA,DIPARTIMENTO PATOL SPERIMENTALE,SEZ PATOL GEN,VIA S GIACOMO 14,I-40126 BOLOGNA,ITALY

来源：

BIOLOGY OF THE CELL | 1992年 / 74卷 / 03期

关键词：

RIBOSOMAL GENES; RIBOSOMAL-RNA SYNTHESIS; ELECTRON MICROSCOPY; TOPOISOMERASE-I; CAMPTOTHECIN;

D O I：

10.1016/0248-4900(92)90039-4

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The effects of camptothecin treatment and topoisomerase I inhibition on ribosomal gene structure and function were investigated in TG cells, a human tumour cell line. 90- and 180-min treatments with 25-mu-M camptothecin resulted in an increased DNA fragmentation and decreased activity of topoisomerase I in cell extracts. After 180-min treatment, the incorporation of labelled uridine into total cell RNA was reduced to 39% and the ribosomal RNA synthesis to 10%, as compared to values of control cells. At the ultrastructural level, the nucleolar components appeared to be segregated; after selective DNA staining, with osmium-amine complex, a part of the nucleolar chromatin of treated cells showed the presence of thin, extended DNA filaments, superimposable to those present in control cells.

引用

页码：281 / 286

页数：6

共 34 条

[1] RAPID EMBEDDING OF TISSUES IN LOWICRYL K4M FOR IMMUNOELECTRON MICROSCOPY [J].