ACTIVATION OF TIMP-2/PROGELATINASE-A COMPLEX BY STROMELYSIN

被引：36

作者：

MIYAZAKI, K

UMENISHI, F

FUNAHASHI, K

KOSHIKAWA, N

YASUMITSU, H

UMEDA, M

机构：

[1] Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, Minami-ku, Yokohama, 232

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 185卷 / 03期

关键词：

D O I：

10.1016/0006-291X(92)91705-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Progelatinase A was purified as a complex with TIMP-2 from the conditioned medium of a human glioblastoma cell line. The TIMP-2/progelatinase complex was resistant to the activation by p-aminophenylmercuric acetic acid (APMA), and showed less than 10% of the activity of the TIMP-2-free active enzyme. When the complex was incubated with stromelysin in the presence of APMA, the 64-kDa progelatinase was effectively converted to the 57-kDa mature enzyme, increasing its gelatinolytic activity about 8-fold. These results suggest that stromelysin is a natural activator of TIMP-2-bound progelatinase A. © 1992.

引用

页码：852 / 859

页数：8

共 21 条

[1] A NOVEL METALLOPROTEINASE GENE SPECIFICALLY EXPRESSED IN STROMAL CELLS OF BREAST CARCINOMAS [J].

BASSET, P ;

BELLOCQ, JP ;

WOLF, C ;

STOLL, I ;

HUTIN, P ;

LIMACHER, JM ;

PODHAJCER, OL ;

CHENARD, MP ;

RIO, MC ;

CHAMBON, P .

NATURE, 1990, 348 (6303) :699-704

[2]

CAWSTON TE, 1981, METHOD ENZYMOL, V81, P711

[3]

COLLIER IE, 1988, J BIOL CHEM, V263, P6579

[4] HUMAN 72-KILODALTON TYPE-IV COLLAGENASE FORMS A COMPLEX WITH A TISSUE INHIBITOR OF METALLOPROTEASES DESIGNATED TIMP-2 [J].

GOLDBERG, GI ;

MARMER, BL ;

GRANT, GA ;

EISEN, AZ ;

WILHELM, S ;

HE, CS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (21) :8207-8211

[5]

HOWARD EW, 1991, J BIOL CHEM, V266, P13064

[6]

LIOTTA LA, 1986, CANCER RES, V46, P1

[7] EPIDERMAL GROWTH-FACTOR AND ONCOGENES INDUCE TRANSCRIPTION OF THE SAME CELLULAR MESSENGER-RNA IN RAT FIBROBLASTS [J].

MATRISIAN, LM ;

GLAICHENHAUS, N ;

GESNEL, MC ;

BREATHNACH, R .

EMBO JOURNAL, 1985, 4 (06) :1435-1440

[8] METALLOPROTEINASES AND THEIR INHIBITORS IN MATRIX REMODELING [J].

MATRISIAN, LM .

TRENDS IN GENETICS, 1990, 6 (04) :121-125

[9]

MIYAZAKI K, 1990, CANCER RES, V50, P7758

[10] STROMELYSIN IS AN ACTIVATOR OF PROCOLLAGENASE - A STUDY WITH NATURAL AND RECOMBINANT ENZYMES [J].

MURPHY, G ;

COCKETT, MI ;

STEPHENS, PE ;

SMITH, BJ ;

DOCHERTY, AJP .

BIOCHEMICAL JOURNAL, 1987, 248 (01) :265-268

← 1 2 3 →