A HYDROGEN-PEROXIDE ASSAY BASED ON THE PEROXIDASE-OXIDASE REACTION - NUMERICAL-SIMULATION OF THE REACTION-MECHANISM

被引：6

作者：

BOUTELET, I ^{[1
]}

ALEXANDRE, S ^{[1
]}

VINCENT, JC ^{[1
]}

机构：

[1] UNIV ROUEN,POLYMERES BIOPOLYMERES MEMBRANES LAB,CNRS,URA 500,F-76821 MONT ST AIGNAN,FRANCE

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1994年 / 223卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1994.tb19017.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to explain an assay method for H2O2, based on the horseradish peroxidase/NADH/H2O2 system, we performed a numerical simulation of this complex reaction mechanism. For this purpose, we applied a simplified version of a model designed by Yokota and Yamazaki to our experimental conditions [Yokota, K. & Yamazaki, I. (1977) Biochemistry 16, 1913-1918]. This modification leads to a kinetic behaviour of the system very similar to our experimental results when the presence of superoxide dismutase is simulated. These simulations confirm that hydrogen peroxide concentrations are proportional to the total amount of NADH oxidized. We also showed that superoxide dismutase activity can be determined by this assay method. Moreover, in samples containing superoxide dismutase and hydrogen peroxide, the simulations showed that both superoxide dismutase and hydrogen peroxide can be determined using our assay method.

引用

页码：489 / 496

页数：8

共 24 条

[1] SUSTAINED OSCILLATIONS AND BISTABILITY IN A DETAILED MECHANISM OF THE PEROXIDASE OXIDASE REACTION [J].

AGUDA, BD ;

LARTER, R .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (06) :2167-2174

[2]

ALEXANDRE S, 1992, CR ACAD SCI III-VIE, V315, P85

[3] A NEW MODEL FOR OSCILLATIONS IN THE PEROXIDASE OXIDASE REACTION [J].

ALEXANDRE, S ;

DUNFORD, HB .

BIOPHYSICAL CHEMISTRY, 1991, 40 (02) :189-195

[4]

ALEXANDRE S, 1988, THESIS U PARIS 6 PAR

[5]

BIELSKI BHJ, 1984, METHOD ENZYMOL, V105, P81

[6]

BOUTELET I, 1991, THESIS U ROUEN ROUEN

[7] A METHOD FOR MEASURING H2O2 BASED ON THE POTENTIATION OF PEROXIDATIVE NADPH OXIDATION BY SUPEROXIDE-DISMUTASE AND SCOPOLETIN [J].

DESANDRO, V ;

DUPUY, C ;

RICHERT, L ;

CORDIER, A ;

POMMIER, J .

ANALYTICAL BIOCHEMISTRY, 1992, 206 (02) :408-413

[8] KINETIC STUDY OF REACTION OF HORSERADISH-PEROXIDASE WITH HYDROGEN-PEROXIDE [J].

DOLMAN, D ;

NEWELL, GA ;

THURLOW, MD ;

DUNFORD, HB .

CANADIAN JOURNAL OF BIOCHEMISTRY, 1975, 53 (05) :495-501

[9] COMPUTER-SIMULATION OF SUSTAINED OSCILLATIONS IN PEROXIDASE-OXIDASE REACTION [J].

FEDKINA, VR ;

ATAULLAKHANOV, FI ;

BRONNIKOVA, TV .

BIOPHYSICAL CHEMISTRY, 1984, 19 (03) :259-264

[10] SPECTROPHOTOMETRIC DETERMINATION OF HYDROGEN-PEROXIDE AND ORGANIC HYDROPEROXIDES AT LOW CONCENTRATIONS IN AQUEOUS-SOLUTION [J].

FREW, JE ;

JONES, P ;

SCHOLES, G .

ANALYTICA CHIMICA ACTA, 1983, 155 (DEC) :139-150

← 1 2 3 →