COMPARATIVE-EVALUATION OF MODIFIED M-FC AND M-TEC MEDIA FOR MEMBRANE-FILTER ENUMERATION OF ESCHERICHIA-COLI IN WATER

被引：49

作者：

CIEBIN, BW

BRODSKY, MH

EDDINGTON, R

HORSNELL, G

CHONEY, A

PALMATEER, G

LEY, A

JOSHI, R

SHEARS, G

机构：

[1] ORILLIA PUBL HLTH LAB, ORILLIA, ON, CANADA

[2] ONTARIO MINIST ENVIRONM & ENERGY LAB SERV, ETOBICOKE, ON, CANADA

[3] ONTARIO MINIST ENVIRONM & ENERGY, THUNDER BAY REG LAB, THUNDER BAY, ON, CANADA

[4] ONTARIO MINIST ENVIRONM & ENERGY, LONDON REG LAB, LONDON, ON, CANADA

[5] ONTARIO MINIST ENVIRONM & ENERGY, KINGSTON REG LAB, KINGSTON, ON, CANADA

[6] DURHAM REG ENVIRONM LAB, PICKERING, ON, CANADA

[7] REG MUNICIPAL OTTAWA CARLTON ENVIRONM SERV DEPT, DIV WATER POLLUT CONTROL, GLOUCESTER, ON, CANADA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 11期

关键词：

D O I：

10.1128/AEM.61.11.3940-3942.1995

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two media used to detect fecal coliforms in water by membrane filtration, m-FC and m-TEC, were modified and supplemented with the chromogenic substrate 5-bromo-6-chloro-3-indoyl-beta-D-glucuronide (BCIG) and were compared for quantitative recovery of Escherichia coli. Student's t test of data from 181 water samples of sewage, rivers, lakes, and wells did not demonstrate any statistically significant differences (P = 0.05) in the enumeration of E. coli with these media. Target colonies were confirmed to be E. coli at rates of 98.6 and 97.3% by using FC-BCIG and TEC-BCIG media, respectively. Glucuronidase-negative isolates of E. coli were encountered at the same frequency (6.0%) on both media. This collaborative study demonstrated that either modified basal medium could be used successfully for detection of E. coli in various nontreated waters within 24 h.

引用

页码：3940 / 3942

页数：3

共 12 条

[1]

[Anonymous], 1993, STANDARD METHODS EXA

[2] MEMBRANE-FILTER METHOD FOR ENUMERATING ESCHERICHIA-COLI [J].