NOVEL PHOSPHOTRANSFERASE SYSTEM GENES REVEALED BY BACTERIAL GENOME ANALYSIS - A GENE-CLUSTER ENCODING A UNIQUE ENZYME-I AND THE PROTEINS OF A FRUCTOSE-LIKE PERMEASE SYSTEM

被引：40

作者：

REIZER, J ^{[1
]}

REIZER, A ^{[1
]}

SAIER, MH ^{[1
]}

机构：

[1] UNIV CALIF SAN DIEGO,DEPT BIOL,LA JOLLA,CA 92093

来源：

MICROBIOLOGY-UK | 1995年 / 141卷

关键词：

SUGAR TRANSPORT; PHOSPHOTRANSFERASE SYSTEM; ANAEROBIOSIS; GLYCEROL DEHYDROGENASE; PYRUVATE FORMATE-LYASE;

D O I：

10.1099/13500872-141-4-961

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Previous publications have demonstrated tire presence of a cryptic gene encoding a novel Enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (PTS). Recent Escherichia coli genome sequencing revealed a gene (ptsA) encoding a new Enzyme I homologue in the 89.1-89.3 centisome region. We have analysed this region, and here describe and characterize open reading frames (ORFs) encoding (1) a fused PTS Enzyme I-IIA(Fru) homologue, (2) a glycerol dehydrogenase, (3) a transaldolase homologue, (4) two PTS IIBFru homologues, (5) a PTS IICFru homologue, and (6) homologues of pyruvate formate-lyase and its activating enzyme. Binary comparison scores, multiple alignments and phylogenetic trees establish the families of proteins to which each of the relevant ORFs belong. Identification of the putative products of this gene cluster leads to the proposal that several of the proteins encoded in this region function in anaerobic carbon metabolism.

引用

页码：961 / 971

页数：11

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