SMALL-SUBUNIT (18S) RIBOSOMAL-RNA GENE DIVERGENCE IN THE GENUS SCHISTOSOMA

被引：47

作者：

JOHNSTON, DA

KANE, RA

ROLLINSON, D

机构：

[1] Department of Zoology, The Natural History Museum, London, SW7 5BD, Cromwell Road

来源：

PARASITOLOGY | 1993年 / 107卷

基金：

英国惠康基金;

关键词：

SCHISTOSOMA; SEQUENCE; RIBOSOMAL RNA; 18S; PHYLOGENY; SECONDARY STRUCTURE;

D O I：

10.1017/S0031182000067251

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

An entire 18S rRNA gene sequence from Schistosoma spindale (1990 bases) and partial 18S rRNA gene sequences from S. haematobium (1950 bases) and S. japonicum (1648 bases) have been determined. Together with the previously published sequence for the S. mansoni 18S rRNA gene, these data encompass the 4 recognized Schistosoma species groups. Although Schistosoma 18S rRNA genes are highly conserved, the sequences permit a preliminary molecular phylogeny to be established for the genus. This identifies S. haematobium and S. spindale as sister taxa in a clade with S. mansoni. S. japonicum does not appear to be closely related to this clade. Much of the observed variation occurs within a 'hypervariable' stretch of the gene corresponding to part of the V4 region of 18S rRNA. Despite this variation, the 3 new sequences fit models of 18S rRNA secondary structure predicted from the S. mansoni sequence. The sequences reported in this paper have been submitted to the EMBL Database under accession numbers Z11976 (S. haematobium), Z11590 (S. japonicum) and Z11979 (S. spindale).

引用

页码：147 / 156

页数：10

共 41 条

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