IMMOBILIZED PH GRADIENTS AND REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - A STRATEGY FOR CHARACTERIZATION OF HEMOGLOBIN-VARIANTS WITH ELECTROPHORETIC MOBILITY IDENTICAL TO THAT OF HB-A - THE CASE OF HB SAN-DIEGO

被引：34

作者：

ROCHETTE, J

RIGHETTI, PG

BOSISIO, AB

VERTONGEN, F

SCHNECK, G

BOISSEL, JP

LABIE, D

WAJCMAN, H

机构：

[1] UNIV MILAN, FAC PHARMACEUT SCI, CHAIR BIOCHEM, I-20133 MILAN, ITALY

[2] UNIV MILAN, DEPT BIOMED SCI & TECHNOL, I-20133 MILAN, ITALY

[3] S CARLO BORROMEO HOSP, CLIN CHEM LAB, MILAN, ITALY

[4] HOP UNIV ST PIERRE, SERV CHIM MED, BRUSSELS, BELGIUM

[5] UNIV LIBRE BRUXELLES, FAC SCI, B-1050 BRUSSELS, BELGIUM

来源：

JOURNAL OF CHROMATOGRAPHY | 1984年 / 285卷 / 01期

关键词：

D O I：

10.1016/S0021-9673(01)87744-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The preparative aspects of immobilized pH gradients applied to abnormal Hb is described. As shown with the example of Hb San Diego, this method is successful even with as small a difference in pHi as 0.01 pH unit. For characterization of such netural variants, reversed-phase high-performance liquid chromatography is demonstrated to be a very efficient tool.

引用

收藏

页码：143 / 152

页数：10

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