EFFECTS OF GLUCOSE AND ADENOSINE ON THE ATP CONTENT OF HAMSTER SPERMATIDS

Effects of glucose and adenosine on ATP metabolism were studied using isolated round spermatids from hamsters. The ATP content of the spermatids was strongly decreased after 1 h of incubation of the cells in the presence of 0-1 тм D-glucose. Glucose (1 mM) had no effect during 18 h of incubation in the presence of 12 mM sodium DL-lactate. However, 10 mM glucose caused an almost complete loss of cellular ATP in the presence of lactate. The effect of adenosine was estimated in the absence of glucose with lactate as the energy-yielding substrate. The cellular ATP content was approximately 4 and 8 nmol/106 cells, after 18 h of incubation in the absence and presence of 0-1 тм adenosine, respectively. This two-fold increase was prevented by inhibitors of adenosine uptake and phosphoryl- ation and was slowly reversed after removal of the exogenous adenosine. Treatment of the cells with adenosine had no effect on the energy charge, which was higher than 0-90, and did not alter the cellular cyclic AMP content. The suggestion that the physiological ATP content of the round spermatids is probably stabilized in the region of 4 nmol/106 cells is discussed. © CSIRO 1990.