RAPID MITOCHONDRIAL PROBES FOR ANALYSIS OF POLYMORPHISMS IN FUSARIUM-OXYSPORUM SPECIAL FORMS

被引：3

作者：

BRIDGE, PD

HOPKINSON, LA

RUTHERFORD, MA

机构：

[1] International Mycological Institute, Egham, Surrey

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1995年 / 21卷 / 03期

关键词：

D O I：

10.1111/j.1472-765X.1995.tb01040.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A method is described for the production of simple mitochondrial DNA probes from filamentous fungi for the partial characterization of mitochondrial DNA without the need for cloning, gradient centrifugation or PCR amplification. A probe (P449) consisting of a 3.38 kb mitochondrial fragment from an isolate of Fusarium oxysporum special form cubense was used to determine RFLPs in restriction digests of total DNA from 28 isolates of F. oxysporum from a variety of hosts and locations. The probe showed mtDNA polymorphisms within and between different special forms.

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页码：198 / 201

页数：4

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