CRYSTALLOGRAPHIC CHARACTERIZATION OF A CU,ZN SUPEROXIDE-DISMUTASE FROM PHOTOBACTERIUM-LEIOGNATHI

被引：10

作者：

REDFORD, SM ^{[1
]}

MCREE, DE ^{[1
]}

GETZOFF, ED ^{[1
]}

STEINMAN, HM ^{[1
]}

TAINER, JA ^{[1
]}

机构：

[1] YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT BIOCHEM,BRONX,NY 10461

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1990年 / 212卷 / 03期

关键词：

D O I：

10.1016/0022-2836(90)90323-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Crystals of a copper-zinc Superoxide dismutase from Photobacterium leiognathi, a luminescent marine bacterium that is the species-specific symbiont of the ponyfish, have been obtained from 2-methyl-2,4-pentanediol solutions. The space group was determined using screenless small-angle precession photographs, and was confirmed by analyzing area detector diffraction data with the XENGEN programs for indexing and refinement. The crystals are monoclinic, space group C2 (a = 126.4 A ̊, b = 87.0 A ̊, c = 44.4 A ̊, β = 92.8 Å), and have two 32,000 Mr dimers per asymmetric unit. The crystals diffract to at least 2.7 Å resolution, are resistant to radiation damage, and are suitable for determination of the structure by X-ray diffraction. © 1990.

引用

页码：449 / 451

页数：3

共 26 条

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