DIFFERENT SHAKE FLASK CLOSURES ALTER GAS-PHASE COMPOSITION AND AJMALICINE PRODUCTION IN CATHARANTHUS-ROSEUS CELL-SUSPENSIONS

被引：37

作者：

LEE, CWT ^{[1
]}

SHULER, ML ^{[1
]}

机构：

[1] CORNELL UNIV,SCH CHEM ENGN,ITHACA,NY 14853

来源：

BIOTECHNOLOGY TECHNIQUES | 1991年 / 5卷 / 03期

关键词：

D O I：

10.1007/BF00152776

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The type of closure chosen for plant cell cultures can significantly alter the headspace gas composition of a culture, leading to major differences in the production of secondary metabolites. In cell suspension cultures of Catharanthus roseus, ethylene accumulated in cultures with limited gas exchange and appeared to inhibit the production of ajmalicine. The variability in product yields between replicates can also be attributed to gas composition differences.

引用

页码：173 / 178

页数：6

共 6 条

[1]

ASADA M, 1989, APPL MICROBIOL BIOT, V30, P475, DOI 10.1007/BF00263851

[2] ETHYLENE GENERATION AND REVERSAL OF ETHYLENE EFFECTS DURING DEVELOPMENT INVITRO OF RAPID-CYCLING BRASSICA-CAMPESTRIS L [J].