LOCALIZATION, CHARACTERIZATION AND ACTIVITY OF PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE IN THE FROG ADRENAL-GLAND

被引:50
作者
YON, L
FEUILLOLEY, M
CHARTREL, N
ARIMURA, A
FOURNIER, A
VAUDRY, H
机构
[1] UNIV ROUEN, EUROPEAN INST PEPTIDE RES,MOLEC ENDOCRINOL LAB, CNRS,URA 650,INSERM,UA, F-76821 MONT ST AIGNAN, FRANCE
[2] TULANE UNIV, HEBERT CTR, US JAPAN BIOMED RES LABS, BELLE CHASSE, LA 70037 USA
[3] UNIV QUEBEC, INST NATL RECH SCI SANTE, POINTE CLAIRE H9R 1G6, PQ, CANADA
关键词
D O I
10.1677/joe.0.1390183
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Pituitary adenylate cyclase-activating polypeptide (PACAP) has recently been isolated from the frog brain and the sequence of the peptide appears to be strikingly similar to that of mammalian PACAP. In the present study, we have investigated the localization of PACAP in the frog interrenal (adrenal) gland by immunocytochemistry using antisera directed against PACAP 38 or PACAP 27. Two types of PACAP-immunoreactive fibres were observed: thick varicose fibres coursing between adrenal cells and thin processes located in the walls of blood vessels irrigating the gland. Bilateral transection of the splanchnic nerves did not affect the intensity and distribution of PACAP immunoreactivity. The mean +/- S.E.M. concentration of PACAP, measured by radioimmunoassay in crude adrenal extracts, was 0.65 +/- 0.16 nmol/g wet tissue. Two molecular forms of PACAP in the adrenal gland were characterized by reversed phase high-performance liquid chromatography combined with radioimmunoassay quantification. The elution profiles revealed the existence of two peaks exhibiting the same retention times as synthetic frog PACAP 38 (fPACAP 38) and PACAP 27, the predominant form being PACAP 38. The possible involvement of PACAP in the regulation of adrenal steroidogenesis was investigated in vitro using a perifusion system for frog adrenal slices. Graded doses of fPACAP 38 (0.1-10 mu mol/l) increased the secretion of both corticosterone and aldosterone in a dose-dependent manner. Administration of repeated pulses of fPACAP 38 (1 mu mol/l), at 120-min intervals, led to a reproducible stimulation of corticosteroid secretion without any tachyphylaxis. Prolonged infusion (2 h) of the peptide induced a rapid increase in corticosterone and aldosterone output, followed by a gradual decline in the secretion rate, suggesting the occurrence of a desensitization phenomenon. Synthetic porcine vasoactive intestinal peptide, which is structurally related to PACAP, was about ten times less potent than fPACAP 38 in stimulating steroidogenesis while the [Des-His(1)]-fPACAP 38 analogue was 100 times less effective. These results demonstrate that a peptide closely related to fPACAP 38 is present in fibres innervating the frog adrenal gland and could participate in the regulation of corticosteroid secretion, particularly during neurogenic stress.
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页码:183 / +
页数:1
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