IDENTIFICATION OF 2 CRITICAL BASE PAIRINGS IN 5' UNTRANSLATED REGIONS AFFECTING TRANSLATION EFFICIENCY OF SYNTHETIC UNCAPPED GLOBIN MESSENGER-RNAS

被引：5

作者：

FRANCES, V ^{[1
]}

MORLE, F ^{[1
]}

GODET, J ^{[1
]}

机构：

[1] UNIV LYON 1, CTR GENET MOLEC & CELLULAIRE, CNRS, UMR 106, 43 BLVD 11 NOVEMBRE 1918, F-69622 VILLEURBANNE, FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1130卷 / 01期

关键词：

TRANSLATION EFFICIENCY; MESSENGER RNA; GLOBIN; BASE PAIR; UNTRANSLATED REGION;

D O I：

10.1016/0167-4781(92)90457-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We observed a marked difference between the in vitro translation efficiency of two uncapped synthetic mRNAs, displaying the entire human alpha or beta-globin mRNA sequences and some additional non-globin sequences in 5'. The comparison of the translation efficiencies of chimeric mRNAs indicated that the alpha-5' untranslated region (5' UTR) is responsible for a low translation efficiency that cannot be explained neither by primary sequence nor by the overall stability of 5' UTR secondary structures only. By point mutations in this alpha-5' UTR, we identified two base pairings at position -1 and -2 preceding the initiation codon which are associated with a negative effect on translation efficiency.

引用

页码：29 / 37

页数：9

共 40 条

[1] GLOBIN CHAIN ELECTROPHORESIS - A NEW APPROACH TO THE DETERMINATION OF THE G-GAMMA/A-GAMMA RATIO IN FETAL HEMOGLOBIN AND TO STUDIES OF GLOBIN-SYNTHESIS [J].