CELL-FREE TRANSLATION SYSTEMS FROM DIFFERENT EUKARYOTES DIFFER IN THEIR SENSITIVITY TO A TEMPLATE SUGAR-PHOSPHATE BACKBONE

被引：3

作者：

POTAPOV, AP ^{[1
]}

OVCHARENKO, GV ^{[1
]}

SOLDATKIN, KO ^{[1
]}

SHULGA, NI ^{[1
]}

SOLDATKIN, AP ^{[1
]}

ELSKAYA, AV ^{[1
]}

机构：

[1] ACAD SCI UKSSR,INST MOLEC BIOL & GENET,ZABOLOTNOGO 150,KIEV 252627,UKRAINE,USSR

来源：

BIOCHIMIE | 1992年 / 74卷 / 05期

关键词：

EUKARYOTIC CELL-FREE TRANSLATION SYSTEM; SENSITIVITY; TEMPLATE SUGAR-PHOSPHATE BACKBONE; POLY(U); POLY(DT);

D O I：

10.1016/0300-9084(92)90083-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To study the role of a messenger sugar-phosphate backbone in the ribosomal decoding process, poly(U) and poly(dT) template activity in different eukaryotic systems has been compared. 80S ribosomes from Saccharomyces cerevisiae appeared to be able to translate poly(dT) both in the presence and in the absence of elongation factors, contrary to poly(U). However, ribosomes from higher eukaryotes (wheat germ, rabbit liver) are completely inefficient in poly(dT) translation. Moreover, rabbit liver ribosomes fail to bind effectively phenylalanyl-tRNA in the presence of poly(dT) although the polynucleotide seems to interact with the ribosomal decoding center. It is also of particular interest that hybrid ribosomes formed from the yeast and rabbit liver subunits can translate poly(dT) only when the large ribosomal subunit from yeast is used.

引用

页码：435 / 441

页数：7

共 26 条

[1]

BIELKA H, 1982, EUKARYOTIC RIBOSOME, P303

[2]

BLACKBURN P, 1977, J BIOL CHEM, V252, P5904

[3] PROTEIN-SYNTHESIS BY HYBRID RIBOSOMES RECONSTRUCTED FROM RABBIT RETICULOCYTE RIBOSOMAL CORE-PARTICLES AND AMPHIBIAN OR FUNGAL SPLIT-PROTEINS [J].

COX, RA ;

GREENWELL, P .

BIOCHEMICAL JOURNAL, 1980, 186 (03) :861-872

[4]

DAMERAU W, 1986, BIOMED BIOCHIM ACTA, V45, P727

[5] STRUCTURE AND FUNCTION OF MAMMALIAN RIBOSOMES .1. ISOLATION AND CHARACTERIZATION OF ACTIVE LIVER RIBOSOMAL SUBUNITS [J].

FALVEY, AK ;

STAEHELI.T .

JOURNAL OF MOLECULAR BIOLOGY, 1970, 53 (01) :1-&

[6] FACTOR-FREE (NON-ENZYMIC) AND FACTOR-DEPENDENT SYSTEMS OF TRANSLATION OF POLYURIDYLIC ACID BY ESCHERICHIA-COLI RIBOSOMES [J].

GAVRILOVA, LP ;

KOSTIASHKINA, OE ;

KOTELIANSKY, VE ;

RUTKEVITCH, NM ;

SPIRIN, AS .

JOURNAL OF MOLECULAR BIOLOGY, 1976, 101 (04) :537-552

[7]

IBRAHIM NG, 1974, J BIOL CHEM, V249, P6806

[8]

MARIBONA R, 1979, Biokhimiya, V44, P1701

[9] PHYSICAL-CHEMICAL PROPERTIES OF STABLE YEAST RIBOSOMES AND RIBOSOMAL-SUBUNITS [J].

MAZELIS, AG ;

PETERMANN, ML .

BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 312 (01) :111-121

[10]

MICHOT B, 1990, EUR J BIOCHEM, V188, P212

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