A SIMPLE AND RAPID TECHNIQUE TO PROCESS FORMALIN-FIXED, PARAFFIN-EMBEDDED TISSUES FOR THE DETECTION OF VIRUSES BY THE POLYMERASE CHAIN-REACTION

被引：36

作者：

KIENE, P

MILDELANGOSCH, K

RUNKEL, M

SCHULZ, K

LONING, T

机构：

[1] UNIV HAMBURG, KRANKENHAUS EPPENDORF,INST PATHOL,DEPT PATHOL, MARTINISTR 52, W-2000 HAMBURG 20, GERMANY

[2] UNIV HAMBURG, KRANKENHAUS EPPENDORF, OBSTET CLIN, DEPT GYNAECOL PATHOL & ELECTRON MICROSCOPY, W-2000 HAMBURG 20, GERMANY

[3] UNIV HAMBURG, KRANKENHAUS EPPENDORF, GYNECOL CLIN, W-2000 HAMBURG 20, GERMANY

来源：

VIRCHOWS ARCHIV A-PATHOLOGICAL ANATOMY AND HISTOPATHOLOGY | 1992年 / 420卷 / 03期

关键词：

POLYMERASE CHAIN REACTION; HUMAN PAPILLOMAVIRUS; CYTOMEGALOVIRUS; EPSTEIN-BARR VIRUS;

D O I：

10.1007/BF01600280

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

The use of chelating resin in a simple, rapid and efficient pre-treatment protocol to process formalin-fixed, paraffin-embedded specimens for the polymerase chain reaction (PCR) is described and compared to other pre-treatment techniques. With this modified PCR protocol, a variety of human autopsy and biopsy specimens were investigated for presence of DNA of human papillomaviruses, cytomegalovirus or Epstein-Barr virus. These viruses were detected in a productive or non-/low-productive state. Amplimers generated ranged from 88 to 450 base pairs length. Under the specified technical conditions, a considerable range of DNA molecules can be amplified from paraffin-embedded material, some older than 10 years.

引用

页码：269 / 273

页数：5

共 21 条

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[2]

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