THE EFFECT OF SUBSTRATE ON THE MOLECULAR-WEIGHT OF POLY-BETA-HYDROXYBUTYRATE PRODUCED BY AZOTOBACTER-VINELANDII UWD

被引：39

作者：

CHEN, GQ

PAGE, WJ

机构：

[1] Department of Microbiology, University of Alberta, Edmonton, T6G 2E9, Alberta

来源：

BIOTECHNOLOGY LETTERS | 1994年 / 16卷 / 02期

关键词：

D O I：

10.1007/BF01021663

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Azotobacter vinelandii UWD produced very high molecular weight (MW) (approx. 4 million Daltons) poly-beta-hydroxybutyrate (PHB) when grown in 5% w/v beet molasses medium. The polymer MW decreased as the beet molasses concentration was increased. Similar results were obtained in equivalent concentrations of sucrose (as raw sugar), but the polymer MW was not greater than 1.6 million. This difference was not caused by more severe oxygen-limitation in the beet molasses medium. It appeared that the non-sugar components of beet molasses promoted the formation of higher MW polymer. Fish peptone, a known PHB-yield-promoter in this organism, did not promote the formation of very high MW polymer.

引用

页码：155 / 160

页数：6

共 15 条

[1] OCCURRENCE, METABOLISM, METABOLIC ROLE, AND INDUSTRIAL USES OF BACTERIAL POLYHYDROXYALKANOATES [J].