CYTOPLASMIC PH REGULATES ATP-INDUCED CA(2+)-DEPENDENT K+-CURRENT OSCILLATION IN RAT MEGAKARYOCYTES

被引：14

作者：

UNEYAMA, C ^{[1
]}

UNEYAMA, H ^{[1
]}

TAKAHASHI, M ^{[1
]}

AKAIKE, N ^{[1
]}

机构：

[1] TOHOKU UNIV,SCH MED,DEPT NEUROPHYSIOL,SENDAI,MIYAGI 980,JAPAN

来源：

BIOCHEMICAL JOURNAL | 1993年 / 295卷

关键词：

D O I：

10.1042/bj2950317

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of cytoplasmic pH (pH(i)) on ATP-induced Ca2+ oscillation of rat megakaryocytes was investigated by the whole-cell patch-clamp technique. Megakaryocytes responded to extracellular ATP and showed the periodic activation of K+ channels, which reflects the oscillation in intracellular free Ca2+ concentration. Intracellular alkalinization by 20 mM NH4Cl resulted in inhibition of the oscillatory response, and intracellular acidification by 20 mM sodium acetate enhanced the response. NH4Cl also inhibited the Ins(1,4.5)P3-induced oscillation. Sodium acetate had no effect on the InsP3-induced oscillation, but enhanced the guanosine 5'-[gamma-thio]triphosphate-induced response. These results indicate that pH, modulates the ATP-induced cellular response at least at two points.

引用

页码：317 / 320

页数：4

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