MULTIPLE GENE TRANSCRIPTS OF THE SOMATOSTATIN RECEPTOR SSTR2 - TISSUE SELECTIVE DISTRIBUTION AND CAMP REGULATION

被引:147
作者
PATEL, YC [1 ]
GREENWOOD, M [1 ]
KENT, G [1 ]
PANETTA, R [1 ]
SRIKANT, CB [1 ]
机构
[1] ROYAL VICTORIA HOSP,DEPT NEUROL & NEUROSURG,MONTREAL H3A 1A1,QUEBEC,CANADA
关键词
D O I
10.1006/bbrc.1993.1412
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The rodent SSTR2 mRNA has been reported to be alternatively spliced to generate long (SSTR2A) and short (SSTR2B) receptor isoforms which differ in sequence at their C-terminal regulatory domains. By extending the 3' nucleotide sequence of the human gene (hSSTR2) we show highly conserved intron/exon boundaries suggesting that hSSTR2 is also capable of generating spliced variants. mRNA blots of rat tissues reveal 2 transcripts of 2.8 and 2.3 kb that are differentially expressed in brain regions and multiple peripheral organs. The 2.3 kb mRNA is preferentially expressed in pituitary tumor cells (AtT-20 mouse, GH3 rat, human prolactinoma, human somatotroph adenoma), but not in rat or human insulinoma cells. This transcript shows 4 fold induction by forskolin in AtT-20 cells suggesting cAMP dependent control of SSTR2 gene expression. The abundant expression of SSTR2 gene, the occurrence of 2 isoforms and evidence of extensive regulation at both gene and peptide levels, suggests that SSTR2 is the principal SST-14 selective subtype. © 1993 Academic Press, Inc.
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页码:288 / 294
页数:7
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