CLONING OF A CANDIDA-ALBICANS PEPTIDE-TRANSPORT GENE

被引:31
作者
BASRAI, MA
LUBKOWITZ, MA
PERRY, JR
MILLER, D
KRAINER, E
NAIDER, F
BECKER, JM
机构
[1] UNIV TENNESSEE,DEPT MICROBIOL,KNOXVILLE,TN 37996
[2] UNIV TENNESSEE,CELLULAR MOLEC & DEV BIOL PROGRAM,KNOXVILLE,TN 37996
[3] CUNY COLL STATEN ISL,DEPT CHEM,STATEN ISL,NY 10301
来源
MICROBIOLOGY-UK | 1995年 / 141卷
关键词
CANDIDA ALBICANS; PEPTIDE TRANSPORT; SACCHAROMYCES CEREVISIAE;
D O I
10.1099/13500872-141-5-1147
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A Candida albicans peptide transport gene, CaPTR2, was cloned from a C. albicans genomic library by functional complementation of a peptide transport deficient mutant (strain ptr2-2) of Saccharomyces cerevisiae. CaPTR2 restored peptide transport to transformants as determined by uptake of radiolabelled dileucine, growth on dipeptides as sources of required amino acids, and restoration of growth inhibition by toxic peptides. Plasmid curing experiments demonstrated that the peptide transport phenotype was plasmid borne. CaPTR2 was localized to chromosome R of C. albicans by contour-clamped homologous electric field gel chromosome blots. Deletion subclones and frameshift mutagenesis were used to narrow the peptide transport complementing region to a 5.1 kb DNA fragment. DNA sequencing of the complementing region identified an ORF of 1869 bp containing an 84 nucleotide intron. The deduced amino acid sequence predicts a protein of 70 kDa consisting of 623 amino acids with 12 hydrophobic segments. A high level of identity was found between the predicted protein and peptide transport proteins of S. cerevisiae and Arabidopsis thaliana. This study represents the first steps in the genetic characterization of peptide transport in C. albicans and initiates a molecular approach for the study of drug delivery against this pathogen.
引用
收藏
页码:1147 / 1156
页数:10
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