CASCADE-LIKE EXPONENTIAL SUBSTRATE AMPLIFICATION IN ENZYME SENSORS

被引：16

作者：

PFEIFFER, D

SCHELLER, FW

MCNEIL, CJ

SCHULMEISTER, T

机构：

[1] MAX DELBRUCK CTR MOLEC MED,BIOSENSORS PROJECT GRP,D-13122 BERLIN,GERMANY

[2] UNIV NEWCASTLE UPON TYNE,SCH MED,DEPT CLIN BIOCHEM,NEWCASTLE TYNE NE2 4HH,TYNE & WEAR,ENGLAND

[3] UNIV POTSDAM,INST COMP INTEGRATED ENGN,D-14476 POTSDAM,GERMANY

来源：

BIOSENSORS & BIOELECTRONICS | 1995年 / 10卷 / 1-2期

关键词：

EXPONENTIAL AMPLIFICATION; ADP ATP SENSOR; MULTIENZYME ELECTRODE; MATHEMATICAL MODELING;

D O I：

10.1016/0956-5663(95)96804-8

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A mathematical model has been developed describing non-linear substrate amplification in amperometric enzyme membrane electrodes. The coupled enzyme reactions of myokinase and pyruvate kinase have been used to realize an exponential amplification system. Using photometric analysis, the lower detection limit obtained via lactate dehydrogenase and NADH detection was 1.5 nM ADP and the expected exponential shape of the signal-time dependency has been observed. The combination of myokinase and pyruvate oxidase in an immobilized form with a pyruvate oxidase electrode resulted in an amplification factor of 800.

引用

页码：169 / 180

页数：12

共 18 条

[1]

Bergmeyer H. U., 1983, METHODEN ENZYMATISCH, V2, P248

[2]

HAGER LP, 1955, METHOD ENZYMOL, P482

[3] EXPLOITATION OF THE FLOW-INJECTION APPROACH FOR ANALYTICAL PROCEDURES BASED ON ENZYMATIC AMPLIFICATION REACTIONS [J].