DETECTION AND QUANTIFICATION OF VIRUS-DNA IN PLASMA OF PATIENTS WITH EPSTEIN-BARR VIRUS-ASSOCIATED DISEASES

被引:106
作者
YAMAMOTO, M [1 ]
KIMURA, H [1 ]
HIRONAKA, T [1 ]
HIRAI, K [1 ]
HASEGAWA, S [1 ]
KUZUSHIMA, K [1 ]
SHIBATA, M [1 ]
MORISHIMA, T [1 ]
机构
[1] TOKYO MED & DENT UNIV, MED RES INST,DEPT CELL REGULAT, DIV VIROL & IMMUNOL,BUNKYO KU, TOKYO 113, JAPAN
关键词
D O I
10.1128/JCM.33.7.1765-1768.1995
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Epstein-Barr virus (EBV) causes various diseases, such as infectious mononucleosis (IM), fatal IM, EBV-associated hemophagocytic syndrome (EBVAHS), and chronic active EBV infection (CAEBV), In the present study, cell-free EBV DNA was detected in the plasma of patients with EBV-associated diseases by PCR assay. The patients included 20 patients with IM, 2 patients with fatal IM, 4 patients with EBVAHS, 4 patients with CAEBV, and 38 healthy children (20 EBV seropositive and 18 EBV seronegative). In patients with IM, plasma samples were positive for EBV DNA in all patients (100%) in the acute phase and in 44% of the patients in the convalescent phase, but plasma samples from tire 38 healthy control children were negative (0%) fdr EBV DNA. Quantitative PCR assay revealed that plasma from patients with IM contained the highest amount of vii us DNA within 7 days following the onset of disease (mean, 6 x 10(4) copies per mi), The EBV DNA concentration decreased thereafter as the patients recovered, Plasma from patients with fatal IM contained more than 100 times more copies of EBV DNA (3 x 10(7) copies per mi) than plasma from patients with IM. Plasma from patients with the acute phase of EBVAHS contained 10 times more copies of EBV DNA (5 x 10(5) copies per mi) than plasma from IM, and then patients with the number of copies decreased similarly in both groups of patients in the convalescent phase (2 x 10(4) copies per mi). The amount of virus DNA in patients with CAEBV (6 x 10(4) copies per mi) was similar to that noted in patients with IM; however, it became higher (1 x 10(6) copies per mi) when the patients' clinical status deteriorated, These data suggest that the presence of cell-free EBV DNA in plasma is a common phenomenon in patients with EBV-associated diseases, The concentration of EBV DNA in plasma seems to be higher in patients with the more severe clinical categories of EBV diseases.
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页码:1765 / 1768
页数:4
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