USE OF SIALYLATED OR SULFATED DERIVATIVES AND ACRYLAMIDE COPOLYMERS OF GAL-BETA-1,3GALNAC-ALPHA- AND GALNAC-ALPHA- TO DETERMINE THE SPECIFICITIES OF BLOOD-GROUP T-SPECIFIC AND TN-SPECIFIC LECTINS AND THE COPOLYMERS TO MEASURE ANTI-T AND ANTI-TN ANTIBODY-LEVELS IN CANCER-PATIENTS

被引:32
作者
CHEN, YX [1 ]
JAIN, RK [1 ]
CHANDRASEKARAN, EV [1 ]
MATTA, KL [1 ]
机构
[1] ROSWELL PK CANC INST, DEPT GYNECOL ONCOL, BUFFALO, NY 14263 USA
关键词
BOAST CANCER; T-; TN-SPECIFIC LECTINS; AGARICUS BISPORUS; HELIX POMATIA; VICIA VILLOSA; ANTIBODIES;
D O I
10.1007/BF00731869
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Sialylated or sulfated derivatives and acrylamide copolymers of blood group T-(Gal beta 1,3GalNAc alpha-) and Tn-(GalNAc alpha) haptens were studied for their interaction with the lectins of peanut (PNA), Agaricus bispovus-(ABA), Helix pomatia-(HPA) acid Vicia villosa B-4-(VVA), using asialo Cowper's gland mucin (ACGM), which contains both T and Tn epitopes, as the coating substrate in enzyme linked lectin assay. Both T and Tn copolymers (similar to 40 haptens) showed high affinity and strict specificity; although the T-copolymer at 0.05-0.07 mu M concentration caused 50% inhibition of interaction of either PNA or ABA with ACGM, there was little inhibition of the HPA and VVA interactions even at over 100 times that concentration. The Tn-copolymer at 0.02-0.05 mu M inhibited HPA or VVA interaction with ACGM by 50% but gave virtually no inhibition of PNA and ABA binding. Sialyl, sulfate or methyl group substitution on C-6 of GalNAc of the T-haptene did not prevent interaction with PNA but almost abolished interaction with ABA. In contrast, sialyl or sulfate group on C-6 and sulfate on C-3 of Gal in Gal beta 1,3GalNAc alpha- inhibited almost completely the interaction of PNA with ACGM but had only a slight effect on the interaction of ABA; C-6 substitution with either sialic acid or sulfate on GalNAc alpha- almost abolished the interaction of both HPA and VVA with ACGM. Preliminary studies revealed a significant depression in the serum level of anti-T (two to three-fold decrease) and anti-Tn (similar to two-fold decrease) antibodies in breast cancer compared with normal control subjects when the acrylamide T- and Tn-copolymers were used as coating substrates in enzyme linked immunoassays.
引用
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页码:55 / 62
页数:8
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