PEMPHIGUS ANTIBODIES AND SHEDDING SNAKE SERUM ENHANCE SUSCEPTIBILITY OF EPIDERMAL-KERATINOCYTES IN NATURAL CYTOTOXIC REACTIONS

被引：4

作者：

GRANDO, SA

VASILYEV, AN

KOSTROMIN, AP

机构：

[1] Medical and Biological Division of Scientific, Industrial Center Technobascom Kiev

[2] Immunodermatology Unit, Chair of Dermato-Venereology, Kiev State Advanced Training Institute for Doctors, Kiev

来源：

AUTOIMMUNITY | 1990年 / 8卷 / 01期

关键词：

NATURAL CYTOTOXICITY; EPIDERMAL KERATINOCYTES; PEMPHIGUS VULGARIS ANTIGEN; PEMPHIGUS VULGARIS ANTIBODIES; SHEDDING SNAKE SERUM PROTEIN;

D O I：

10.3109/08916939008998427

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In order to clarify the role of natural cytotoxicity (NC) in the damage of epidermal keratinocytes (EK) in pemphigus vulgaris (PV) we used the direct 51Cr-release assay in studying the cytotoxic activity of large grandular lymphocytes (LGL), obtained from 34 acute PV patients and 19 healthy donors against EK of PV patients, donors, intact newborn BALB/c mice and mice with experimental PV, shedding and non-shedding grass snakes. We also investigated the effect of pemphigus antibodies and shedding snake serum protein (SSSP) upon EK sensititivity to cytotoxic effects of NC effectors. In other experiments, studied were the amount of serine proteinases secreted by donor LGL in the presence of membrane antigen of antibody-or SSSP-transformed EK, and direct effect of pemphigus antibodies and SSSP upon the release of radioactive label and endoproteinases from EK. It was found that EK, which had interacted with pemphigus antibodies or SSSP, became a sensitive target for NC effectors. Membrane antigen of antibody-and SSSP-transformed human, murine and snake EK induces the secretion of endproteinases by NC effector cells. The conclusion was made that the effect of both pemphigus antibodies and SSSP on EK resulted in the emergences of a new antigen on EK cellular membrane which is recognized by NC effectors and plays the role of the ligand in receptor/ligand interaction between LGL and their targets. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：9 / 16

页数：8

共 37 条

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