EVIDENCE OF GENE INTROGRESSION IN APPLE USING RAPD MARKERS

被引：28

作者：

DURHAM, RE ^{[1
]}

KORBAN, SS ^{[1
]}

机构：

[1] UNIV ILLINOIS,DEPT HORT,URBANA,IL 61801

来源：

EUPHYTICA | 1994年 / 79卷 / 1-2期

关键词：

APPLE; MALUS; GENE INTROGRESSION; RAPDS; PCR; DNA MARKERS; VENTURIA INAEQUALIS; APPLE SCAB RESISTANCE;

D O I：

10.1007/BF00023582

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A genomic remnant of Malus floribunda clone 821 introgressed into the cultivated apple M. x domestica Borkh. was identified using randomly amplified polymorphic DNA (RAPD) markers obtained by the polymerase chain reaction (PCR). Using a set of 59 oligonucleotide decamer primers, polymorphic DNA markers were identified among three pooled DNA samples. Based on the presence or absence of bands among bulked apple scab-resistant selections and cultivars, bulked scab-susceptible cultivars, and a M. floribunda clone 821 sample, one primer, A15, identified amplified fragments in the scab-resistant bulked sample that was also unique to the M. floribunda clone 821. The unique band from M. floribunda clone 821 was amplified in four out of 17 scab-resistant selections/cultivars. This RAPD, designated OA15(900), identifies an introgressed fragment that has as yet no known function.

引用

页码：109 / 114

页数：6

共 21 条

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