IRREVERSIBLE BINDING-KINETICS OF BACILLUS-THURINGIENSIS CRYIA DELTA-ENDOTOXINS TO GYPSY-MOTH BRUSH-BORDER MEMBRANE-VESICLES IS DIRECTLY CORRELATED TO TOXICITY

被引：75

作者：

LIANG, YZ ^{[1
]}

PATEL, SS ^{[1
]}

DEAN, DH ^{[1
]}

机构：

[1] OHIO STATE UNIV, DEPT BIOCHEM, COLUMBUS, OH 43210 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1995年 / 270卷 / 42期

关键词：

D O I：

10.1074/jbc.270.42.24719

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To examine the binding of Bacillus thuringiensis delta-endotoxins, CryIAa, CryIAb, and CryIAc, to Lymantria dispar (gypsy moth) brush border membrane vesicles (BBMV), saturation kinetic analyses were conducted according to a two-step interaction scheme [GRAPHICS] for delta-endotoxin binding to BBMV, rather than the one-step reversible binding presented in prior reports. The order of toxicity of the delta-endotoxins, as measured by the dose required for a 50% inhibition of weight gain (ID50), was CryIAa (77.3 ng) > CryIAb (157 ng) > CryIAc (187 ng). While both the maximum extent of binding, B-max and the half-maximum insertion rate concentration, K-1/2 was observed to be indirectly related to toxicity, the rate constant of irreversible binding, k(2), was found to be directly correlated to toxicity.

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页码：24719 / 24724

页数：6

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