RELATIONSHIP BETWEEN THE CLOSTRIDIUM-PERFRINGENS CATQ GENE-PRODUCT AND CHLORAMPHENICOL ACETYLTRANSFERASES FROM OTHER BACTERIA

The nucleotide sequence of the Clostridium perfringens chloramphenicol acetyltransferase (CAT)-encoding resistance determinant, catQ, was determined. An open reading frame encoding a protein of 219 amino acids with a molecular weight of 26,014 was identified. Although catQ was expressed constitutively, sequences similar in structure to those found upstream of inducible cat genes were observed. The catQ gene was distinct from the C. perfringens catP determinant. The deduced CATQ monomer had considerable amino acid sequence conservation compared with CATP (53% similarity) and other known CAT protein (39 to 53%). Phylogenetic analysis revealed that the CATQ monomer was as closely related to CAT proteins from Staphylococcus aurenus and Campylobacter coli as it was to CAT monomer from the clostridia.