A NA PUMP INHIBITOR FROM BOVINE POSTERIOR PITUITARY - PURIFICATION, STRUCTURE DETERMINATION AND ITS CARDIOVASCULAR EFFECT IN RAT

被引：7

作者：

HAMAKUBO, T

FURUTA, H

ICHIMURA, M

APPALSAMY, M

MOSQUEDAGARCIA, R

ROBERTSON, D

INAGAMI, T

机构：

[1] VANDERBILT UNIV,MED CTR,SCH MED,DEPT MED,NASHVILLE,TN 37232

[2] VANDERBILT UNIV,MED CTR,SCH MED,DEPT PHARMACOL,NASHVILLE,TN 37232

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 189卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)92256-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We examined the hypothesis that hypothalamo-hypophysial tissue contains an endogenous Na pump inhibitor. From bovine posterior pituitary, we purified a substance which inhibits Rb uptake by human erythrocytes. This inhibitory activity was found in the eluate of 10% acetonitrile from a C18 flash column and purified by subsequent three steps of reversed-phase high-performance liquid chromatography (HPLC). Sequence analysis revealed that this substance was identical to joining peptide, one of the major products of proopiomelanocortin (POMC). This peptide had hypertensive and tachycardiac effects in spontaneously hypertensive rats (SHR) after central administration, with weak Na,K-ATPase inhibitory activity (IC50 = 0.5 mM). © 1992.

引用

页码：691 / 696

页数：6

共 20 条

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